Entomopathogenic Fungi Screening against Fruit Fly in Thailand

The screening of entomopathogenic fungi, 7 isolates of Metarhizium spp., 12 isolates of Beauveria spp., and 1 isolate of Hirsutella citriformis against fruit fly was performed in Lampang Agricultural Research and Training Centre, Lampang, Thailand. The efficacy test was done by spraying suspension of 10 spores per ml of fungus on Bactrocera dorsalis in completely randomized design experiments. The numbers of the dead fruit flies which were covered by fungus was recorded and percent mortality was calculated by Abbott’s formula. The results showed that the percentage of fruit fly mortality was from 2 to 68%. Beauveria sp. isolate 6241 which killed 50% of fruit flies was selected and virulent level was checked by calculating the LC50 using completely randomized design experiments. The results showed that the numbers of the fruit flies killed by fungi were correlated with the spore concentration. Its LC50 was 7.36x10spores/ml respectively.